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. 2021 Jan 20;45(3):1023–1032. doi: 10.3892/or.2021.7944

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Copyright: © Ota et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Western blotting and enzyme-linked immune assay of TFPI-2. (A) Western blotting of TFPI-2 expression in 11 cell lines. Vinculin was used for WCF loading control. (B) WCFs were further divided into nuclear and cytoplasm fractions. Lamin A and α-tubulin were used for nucleus and cytoplasm markers, respectively. (C) TFPI-2 concentration in CM was assessed by enzyme-linked immune assay. TFPI-2, tissue factor pathway inhibitor-2; ECF, extracellular fraction; WCF, whole cell fraction; NE, nuclear extract; CP, cytoplasm; CM, conditioned medium.