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. 2021 Jan 8;45(3):1153–1161. doi: 10.3892/or.2021.7929

Figure 4.

Figure 4.

Snail is involved in radiation- and visfatin-induced cell migration. mRNA expression levels of Snail, Slug, Twist and Zeb1 in (A) A549 or (B) H1299 cells treated with 5 Gy radiation (Radiation) or incubated without radiation (Con) for 24 h. (C) Protein expression levels of Snail in A549 or H1299 cells treated with 5 Gy radiation (Radiation) or incubated without radiation (Con) for 24 h. (D and E) Snail mRNA expression levels in (D) A549 and (E) H1299 cells treated with 5 Gy radiation (Radiation) or incubated without radiation (Con) and further incubated with or without 100 ng/ml anti-visfatin antibody for 48 h. (F) Snail and E-Cad protein expression levels in A549 cells transfected with si-NC or si-Snail for 12 h, then further treated with 5 Gy radiation (Radiation) or incubated without radiation (Con) for 24 h. Data are presented as the mean ± SD of three independent experiments. **P<0.01. E-Cad; E-cadherin; Con, control; NS, not significant; si, small interfering; NC, negative control.