Figure 1.

Effect of VSP-17 treatment on the activation of AMPK. (A) MDA-MB-231 and MDA-MB-453 cells were treated with 1, 3 or 10 µM VSP-17 for 24 h. The protein expression levels of p-AMPK and AMPK were analyzed using western blotting. (B) MDA-MB-231 and MDA-MB-453 cells were treated with 10 µM VSP-17 for 6, 12 or 24 h, and the expression levels of p-AMPK and AMPK were analyzed using western blotting. The data are expressed as the mean ± SEM of three independent experiments. *P<0.05, **P<0.01 vs. control. AMPK, AMP-activated protein kinase; p-, phosphorylated.