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. 2020 Dec 30;45(3):975–986. doi: 10.3892/or.2020.7916

Figure 5.

Figure 5.

Effects of GW9662 and siPPARγ on the VSP-17-induced inhibition of the epithelial-mesenchymal transition (EMT) process in MDA-MB-231 and MDA-MB-453 cells. (A and B) Expression levels of E-cadherin and vimentin in MDA-MD-231 and MDA-MD-453 cells treated with 10 µM VSP-17 and/or 1 µM GW9662 (A) and/or transfected with siPPARγ (B) were analyzed using western blotting. (C and D) mRNA expression levels of E-cadherin in MDA-MB-231 and MDA-MB-453 treated with 10 µM VSP-17 and/or 1 µM GW9662 (C) and/or transfected with siPPARγ (D) were analyzed using reverse transcription-quantitative PCR. (E and F) Cells were cultured with 10 µM VSP-17 and/or 1 µM GW9662 (E) and/or transfected with siPPARγ (F) and the expression levels of E-cadherin and vimentin were measured by immunofluorescence. The data are expressed as the mean ± SEM of three independent experiments. **P<0.01 vs. control; &P<0.05, &&P<0.01 vs. VSP-17. si, small interfering RNA; PPARγ, peroxisome proliferator-activated receptor γ.