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. 2021 Jan 22;45(3):997–1010. doi: 10.3892/or.2021.7947

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Copyright: © Ichimiya et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Inhibition of GLI2 suppresses GBC-cell derived tumor proliferation in vivo. (A) BALB/c nude mice were implanted with control siRNA- or GLI2 siRNA-transfected NOZ cells. (B) GLI2 expression in implanted NOZ cells. (C) Representative images of mice implanted with NOZ cells transfected with control siRNA (left) or GLI2 siRNA (right), and tumor volumes in the control siRNA group and GLI2 siRNA group. (D) Immunohistochemical staining of αSMA, MT, Ki-67, and VEGF in resected xenograft tumors. Original magnification is ×200. Positively-stained areas were quantified using ImageJ. Scale bar, 100 µm. *P<0.05, **P<0.01 and ***P<0.001. Bar, mean ± SD. GLI2, glioma-associated oncogene homolog 2; GBC, gallbladder cancer; siRNA or si, small interfering RNA; siCont, control siRNA; siGLI2, GLI2 siRNA; αSMA, α-smooth muscle actin; MT, Masson's Trichrome staining; VEGF, vascular endothelial growth factor.