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. 2021 Feb 3;12:30. doi: 10.1186/s40104-021-00551-9

Table 2.

Alpha diversity. Number of reads per group-treatment assigned to OTUs and alpha diversity metrics values of feed, gut mucosa (GMMC), and skin mucus microbial communities (SMMC) of rainbow trout fed TM 0 and TM 100 diets

Items TM 0 TM 100 P-value
Feed (rarefied at 21,146 reads)
 Reads 54,465 ± 18,561 44,708 ± 19,771 0.498
 Observed OTUs 107 ± 20 84 ± 25 0.248
 Shannon 3.73 ± 0.05 3.29 ± 0.07 0.021
 Pielou’s evenness 0.55 ± 0.02 0.52 ± 0.03 0.083
 Faith PD 7.79 ± 0.68 6.70 ± 1.05 0.149
GMMC (rarefied at 21,146 reads)
 Reads 63,530 ± 31,477 61,665 ± 16,983 0.901
 Observed OTUs 13 ± 3 10 ± 4 0.231
 Shannon 1.32 ± 0.76 0.28 ± 0.24 0.054
 Pielou’s evenness 0.36 ± 0.21 0.09 ± 0.07 0.055
 Faith PD 1.34 ± 0.23 1.26 ± 0.42 0.872
SMMC (rarefied at 16,752 reads)
 Reads 49,824 ± 21,594 39,064 ± 16,875 0.359
 Observed OTUs 187 ± 40 154 ± 71 0.336
 Shannon 4.43 ± 1.05 4.29 ± 1.05 0.749
 Pielou’s evenness 0.59 ± 0.13 0.61 ± 0.06 0.521
 Faith PD 17.30 ± 4.52 13.86 ± 7.18 0.423

All data are expressed as means ± SD (n = 4 for feed and n=6 for GMMC and SMMC). P<0.05 are in bold