Table 2.
Alpha diversity. Number of reads per group-treatment assigned to OTUs and alpha diversity metrics values of feed, gut mucosa (GMMC), and skin mucus microbial communities (SMMC) of rainbow trout fed TM 0 and TM 100 diets
Items | TM 0 | TM 100 | P-value |
---|---|---|---|
Feed (rarefied at 21,146 reads) | |||
Reads | 54,465 ± 18,561 | 44,708 ± 19,771 | 0.498 |
Observed OTUs | 107 ± 20 | 84 ± 25 | 0.248 |
Shannon | 3.73 ± 0.05 | 3.29 ± 0.07 | 0.021 |
Pielou’s evenness | 0.55 ± 0.02 | 0.52 ± 0.03 | 0.083 |
Faith PD | 7.79 ± 0.68 | 6.70 ± 1.05 | 0.149 |
GMMC (rarefied at 21,146 reads) | |||
Reads | 63,530 ± 31,477 | 61,665 ± 16,983 | 0.901 |
Observed OTUs | 13 ± 3 | 10 ± 4 | 0.231 |
Shannon | 1.32 ± 0.76 | 0.28 ± 0.24 | 0.054 |
Pielou’s evenness | 0.36 ± 0.21 | 0.09 ± 0.07 | 0.055 |
Faith PD | 1.34 ± 0.23 | 1.26 ± 0.42 | 0.872 |
SMMC (rarefied at 16,752 reads) | |||
Reads | 49,824 ± 21,594 | 39,064 ± 16,875 | 0.359 |
Observed OTUs | 187 ± 40 | 154 ± 71 | 0.336 |
Shannon | 4.43 ± 1.05 | 4.29 ± 1.05 | 0.749 |
Pielou’s evenness | 0.59 ± 0.13 | 0.61 ± 0.06 | 0.521 |
Faith PD | 17.30 ± 4.52 | 13.86 ± 7.18 | 0.423 |
All data are expressed as means ± SD (n = 4 for feed and n=6 for GMMC and SMMC). P<0.05 are in bold