Figure 3.

Expression levels of circadian clock proteins in the Res cells with PTX treatment. (A) Total extracts of cells were harvested at 2 h post-dexamethasone (0.1 µM). The expression levels of β-Actin and circadian clock proteins, including BMAL1, CLOCK, PER2 and CRY1, were assessed via western blot analysis. (B) EJ and UMUC3 cells were treated with serum-free medium for 24, 48 and 72 h. The expression levels of BMAL1, CLOCK, PER2 and CRY1 were detected using western blotting. Data are presented as the OD fold difference related to the control from three duplicate experiments. *P<0.05. CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1; Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density.