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. 2021 Jan 22;6(4):3140–3148. doi: 10.1021/acsomega.0c05545

Figure 2.

Figure 2

Apoptotic cells during LPS induced inflammatory stress in differentiated Caco-2 cells. Differentiated Caco-2 cells were cultured for 48 h with 50 μM porcine liver or ascidian muscle EtnGpl containing LPS (50 μg/mL) and then stained with TdT-mediated dUTP nick end labeling (TUNEL) immunofluorescence followed by staining with 4′,6-diamidino-2-phenylindole (DAPI). Representative images (objective, 100× ). (a) Blank. (b) LPS (control). (c) LPS + 50 μM porcine liver EtnGpl. (d) LPS + 50 μM ascidian muscle EtnGpl. Scale bar indicates 20 μm. (e) Induction of apoptosis in differentiated Caco-2 cells by LPS. Values represent means ± SEM, n = 3. Different letters indicate significant differences at P < 0.05, determined by ANOVA (Tukey’s test).