Figure 6.
TNF-α levels during LPS induced inflammatory stress in differentiated Caco-2 cells. (a) Differentiated Caco-2 cells were cultured for 24 h with LPS + 50 μM EtnGpl, and in another experiment, differentiated Caco-2 cells were treated with 50 μM EtnGpl for 16 h (pre-treatment) followed by 50 μg/mL of LPS for 24 h (post-treatment after EtnGpl was taken up). Values represent means ± SEM, n = 3. Different letters indicate significant differences at P < 0.05, determined by ANOVA (Tukey’s test). ns, not significant (t test, *P < 0.05).