. 2021 Jan 20;6(4):2824–2835. doi: 10.1021/acsomega.0c05138

Table 4. pK_a Values of Individual Nitrogen Atoms of Paromomycin Determined Using ¹H, ¹³C, and ¹H−¹⁵N HMBC NMR Spectroscopies in this Work and Compared with the Published Data, as Indicated^a^,^b^,^c^,^d.

pK_a values of individual nitrogen atoms of paromomycin free-base determined using ¹⁵N NMR spectroscopy in H₂O/D₂O (85: 15 v/v) relative to NH₃ using 1 M [¹⁵N] urea in DMSO at 25 °C.³¹

pK_a values of individual nitrogen atoms of paromomycin sulfate determined using ¹⁵N NMR spectroscopy in H₂O/D₂O (85:15 v/v) relative to NH₃ using 1 M [¹⁵N] urea in DMSO at 25 °C.²⁹

This work (n = 1).

The pK_a value of N-6‴ of paromomycin determined using ¹H NMR spectroscopy (in this work) is the average pK_a of the values of N-6‴ obtained using ¹H NMR spectroscopic data for 6‴a (9.14) and 6‴b (9.16).

Table 4. pKa Values of Individual Nitrogen Atoms of Paromomycin Determined Using 1H, 13C, and 1H−15N HMBC NMR Spectroscopies in this Work and Compared with the Published Data, as Indicateda,b,c,d.

Table 4. pK_a Values of Individual Nitrogen Atoms of Paromomycin Determined Using ¹H, ¹³C, and ¹H−¹⁵N HMBC NMR Spectroscopies in this Work and Compared with the Published Data, as Indicated^a^,^b^,^c^,^d.