. 2021 Jan 20;6(4):2824–2835. doi: 10.1021/acsomega.0c05138

Table 5. pK_a Values of the Two Guanidine Groups (N-1 and N-3) and the Secondary Amine (N-Methyl) N-2′ of Streptomycin Determined Using ¹H, ¹³C, and ¹H−¹⁵N HMBC NMR Spectroscopies in this Work and Compared with the Published Data, as Indicated^a^,^b.

pK_a values of individual nitrogen atoms of streptomycin determined using ¹H NMR spectroscopy in H₂O/D₂O (95:05 v/v) relative to DSS at 25 °C.³²

This work (n = 1).

Table 5. pKa Values of the Two Guanidine Groups (N-1 and N-3) and the Secondary Amine (N-Methyl) N-2′ of Streptomycin Determined Using 1H, 13C, and 1H−15N HMBC NMR Spectroscopies in this Work and Compared with the Published Data, as Indicateda,b.