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. 2021 Jan 25;10(1):bio054189. doi: 10.1242/bio.054189

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Characterization of HLCs. (A) Representative immunocytochemistry of hepatocyte markers at the end of HLC differentiation for the line CO2. Cells were stained for ALB (red) and AFP (green) (upper lane), ALB (red) and ECAD (green) middle lane, HNF4α (red) (lower lane). DNA was stained with Hoechst 33258. (B) Expression of hepatocyte markers ALB, AFP, CYP3A4, cEBPα, A1AT, and TTR was confirmed by qRT-PCR. Fold change towards iPSCs was calculated and converted into percentage. iPSCs: n=2, HLCs: n=3, PHH and fetal liver RNA: n=1. Data are means +/− 95% confidence interval. Significances in comparison to iPSCs were calculated with unpaired two-tailed Student's t-tests. *=P<0.05, **=P<0.01, ***=P<0.001. (C) CYP3A4 activity in HLCs. n=3, mean values +/− s.d. are shown.