Table 1.

Clinicopathological factors associated with baseline ctDNA level

	Median cfDNA level copies per ml (median,interquartile range)	P value	Mutant copies per ml (median,interquartile range)	P value	Mutant allele fraction %	P value	ctDNA detection	P value
Age
Below 60	184,370 (105,485–264,285)	0.796	370 (185–745)	1	0.2	0.793	2/3 (67%)	1
Above 60	56,330 (43,245–70,015)		60 (15–1395)		0.115		4/6 (67%)
Pathology subtype
Endometroid	64,060 (/)	0.42	60 (/)	0.467	0.09	0.568	1/1 (100%)	0.446
Clear cell	693,400 (/)		1840 (/)		2.65		1/1 (100%)
Serous	45,030 (36,615–150,427.5)		215(15–932.5)		0.17		4/6 (67%)
Dedifferentiated	72,000 (/)		0 (/)		0		0/1 (0%)
FIGO stage
I–II	38,230 (32,900–49,095)	0.05	0 (0–15)	0.017	0.07	0.025	1/4 (25%)	0.025
III–IV	184,370 (64,060–344,200)		1120 (370–1840)		2.65		5/5 (100%)
Tumor size
<  = 5 cm	52,760 (37,745–94,137.5)	0.327	60 (45–137.5)	0.453	0.115	0.455	3/4 (75%)	0.655
> 5 cm	72,000 (48,600–344,200)		1120 (0–1840)		2.65		3/5 (60%)
Depth of invasion
Superficial	38,230 (32,900–77,187.5)	0.086	30 (0–137.5)	0.169	0.07	0.213	2/4 (50%)	0.371
Deep	72,000 (64,060–344,200)		1120 (370–1840)		2.65		4/5 (80%)
Node positive
Yes	184,370 (64,060–344,200)	0.05	1120 (370–1840)	0.017	2.65	0.025	5/5 (100%)	0.025
No	38,230 (32,900–49,095)		0 (0–15)		0.07		1/4 (25%)
LVSI
Yes	128,185 (54,450–304,242.5)	0.142	745 (92.5–1660)	1	1.425	1	2/3 (67%)	0.655
No	41,460 (38,230–52,760)		60 (30–60)		0.09		4/6 (67%)
Involvement of lower uterine segment
Yes	68,030 (52,465–276,150)	0.439	590 (15–1660)	0.51	1.37	0.599	2/3 (67%)	1
No	41,460 (38,230–112,915)		60 (30–215)		0.14		4/6 (67%)

P values for association between clinicopathological factors and baseline ctDNA level (median cfDNA levels, mutant copies, mutant allele fraction and ctDNA detection rate) were determined. Advanced FIGO stage and node metastasis were associated with high level of mutant copies and mutant allele fraction of ctDNA (FIGO stage: p = 0.017/0.025, node status: p = 0.017/0.025) and high detection rate of ctDNA (p = 0.025, both)