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. 2021 Feb 3;21:85. doi: 10.1186/s12935-021-01791-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A novel up-regulated circRNA circ-ANXA7 is an independent prognostic factor for LUAD. a circ-ANXA7 up-regulation was found between LUAD tissues (n = 40) and non-tumor tissues (n = 40) by microarray analysis. Red: up-regulation and green: down-regulation. b Box plots visualizing a higher expression level of circ-ANXA7 in LUAD tissues than non-tumor tissues using qRT-PCR. c qRT-PCR was utilized to examine the relative expression levels of circ-ANXA7 between normal epithelial cells and LUAD cells. d Overall survival analysis between LUAD patients with high circ-ANXA7 expression and those with its low expression. e Multivariate regression analysis of circ-ANXA7 expression after adjusting other prognostic factors. f Western blot was presented to examine the expression of ANXA7 protein between normal epithelial cells and LUAD cells. g Immunohistochemistry of ANXA7 between adjacent normal tissues and LUAD tissues. Magnification: ×40; ×200. **p < 0.01