Figure 3.

PDIA4 blocks ovarian cancer cell invasion and migration. Ovarian cancer cells OVCAR3 and SKOV3 were transfected withpcDNA3.1 or pcDNA3.1‐PDIA4, respectively. A, PDIA4 mRNA expression was determined by RT‐qPCR. B, PDIA4 protein expression in OVCAR3 and SKOV3 cells was detected by Western blot assay. C and D, Viability of OVCAR3 and SKOV3 cells was measured by MTT assay. E and F, Invasion ability of OVCAR3 and SKOV3 cells was detected by Transwell assay. G and H, Migration ability of OVCAR3 and SKOV3 cells was detected by scratch test. All data were expressed as mean ± SD (n = 3). *p < .05, **p < .01, ***p < .001. mRNA, messenger RNA; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyltetrazolium bromide; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction