Figure 4.

miR‐378a‐3p negatively regulates PDIA4. Ovarian cancer cells OVCAR3 and SKOV3 were transfected with miR‐378a‐3p mimic, miR‐378a‐3p inhibitor or negative control, respectively. A, PDIA4 mRNA expression in OVCAR3 and SKOV3 cells was determined by RT‐qPCR. B, PDIA4 protein expression was detected by Western blot assay. C, The online software Starbase was searched to predict the targeting sites of miR‐378a‐3p and PDIA4 and the designed PDIA4 mutation sites. The mimic NC or miR‐378a‐3p mimic, or inhibitor NC or miR‐378a‐3p inhibitor with WT‐PDIA4 or MT‐PDIA4 were transfected or cotransfected into HEK‐293T cells. D, The luciferase activity was detected by dual‐luciferase reporter gene assay. E and F, RIP assay further examined the targeting relationship between miR‐378a‐3p and PDIA4 in OVCAR3 and SKOV3 cells. All data were expressed as mean ± SD (n = 3). *p < .05, **p < .01, ***p < .001. mRNA, messenger RNA; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction