Figure 5.

ZAP70 is dispensable for ligand‐induced TCR downregulation in human CD4⁺ PBLs. Human PBLs were transduced with retroviral nontarget microRNA (dark grey), or microRNA vectors targeting ZAP70 (light grey). After transduction, Ly6G (A), and total ZAP70 (B) expression was measured; representative histograms are depicted. Transduced cells were left unstimulated (−), or stimulated with a low (+) or high (++) dose of anti‐CD3/CD28 and surface TCRβ (C, D) and total TCRζ (E, F) expression was measured. (C–E) Representative histograms where dark grey histograms indicate nontarget microRNAs and light grey histograms indicate ZAP70 microRNAs. (D, F) Each symbol represents an individual donor (n = 3) examined in a separate experiment, with bars depicting the mean and standard deviations. PBL, peripheral blood lymphocytes; TCR, T‐cell receptor. Significance is calculated with the paired Student's t test