Figure 2.

Gating strategy for the intracellular interferon‐α staining of pDCs under TLR‐7 stimulation. (A) Compared to control conditions using phosphate‐buffered saline (PBS), (B) TLR‐7 stimulation increased the portion of pDCs identified as positive for interferon‐α‐PE to 11.9%, (C) the effects of which were reduced to 5.6% by IL‐4 pretreatment. Left panel: Whole cell population. Middle panel: BDCA‐2+ cell population. Right panel: Interferon‐α + staining gating from BDCA2+ cell population. IL‐4, interleukin 4; pDC, plasmacytoid dendritic cell; TRL‐7, toll‐like receptor 7