Table 1. Loop-mediated isothermal amplification primer sequences.
| Primer set | Sequence | Ref. |
|---|---|---|
| E1 | [11] | |
| E1-F3 | TGAGTACGAACTTATGTACTCAT | |
| E1-B3 | TTCAGATTTTTAACACGAGAGT | |
| E1-FIP | ACCACGAAAGCAAGAAAAAGAAGTTCGTTTCGGAAGAGACAG | |
| E1-BIP | TTGCTAGTTACACTAGCCATCCTTAGGTTTTACAAGACTCACGT | |
| E1-LF | CGCTATTAACTATTAACG | |
| E1-LB | GCGCTTCGATTGTGTGCGT | |
| E1-QFIP | /5IABkFQ/ACCACGAAAGCAAGAAAAAGAAGTTCGTTTCGGAAGAGACAG | |
| E1-FD | ACTTCTTTTTCTTGCTTTCGTGGT/3Joe_N/ | |
| IAV | [26] | |
| IAV-F3-1 | GACTTGAAGATGTCTTTGC | |
| IAV-F3-2 | GACTGGAAAGTGTCTTTGC | |
| IAV-B3-1 | TRTTATTTGGGTCTCCATT | |
| IAV-B3-2 | TRTTGTTTGGGTCCCCATT | |
| IAV-FIP | TTAGTCAGAGGTGACARRATTGCAGATCTTGAGGCTCTC | |
| IAV-BIP | TTGTKTTCACGCTCACCGTGTTTGGACAAAGCGTCTACG | |
| IAV-LF | GTCTTGTCTTTAGCCA | |
| IAV-LB | CMAGTGAGCGAGGACTG | |
| IAV-QFIP | /5IAbRQ/TTAGTCAGAGGTGACARRATTGCAGATCTTGAGGCTCTC | |
| IAV-Fd | CAATYYTGTCACCTCTGACTAA/3Cy5Sp/ | |
| IBV | [26] | |
| IBV-F3 | GCAACCAATGCCACCATA | |
| IBV-B3 | TTCTCTCTTCAAGRGACATC | |
| IBV-FIP | TAGTCAAGGGCYCTTTGCCACTTTGAAGCAGGAATTCTGGA | |
| IBV-BIP | CAAGACCGCCTAAACAGACTAAACTTTTACTTTCAGGCTCACTT | |
| IBV-LF | TGAAAGYCTTTCATAGCAC | |
| IBV-LB | CAAGAATAAAGACTCACAAC | |
| IBV-QFIP | /5IABkFQ/TAGTCAAGGGCYCTTTGCCACTTTGAAGCAGGAATTCTGGA | |
| IBV-Fd | TGGCAAAGRGCCCTTGACTA/36-FAM/ | |
| ACTB | ||
| ACTB-F3 | AGTACCCCATCGAGCACG | |
| ACTB-B3 | AGCCTGGATAGCAACGTACA | |
| ACTB-FIP | GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA | |
| ACTB-BIP | CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC | |
| ACTB-LF | TGTGGTGCCAGATTTTCTCCA | |
| ACTB-LB | CGAGAAGATGACCCAGATCATGT | |
| ACTB-QFIP | /5IAbRQ/GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA | |
| ACTB-Fd | TACAATGAGCTGCGTGTGGCTC/3Rox_N/ | |
| N2 | [11] | |
| N2-F3 | ACCAGGAACTAATCAGACAAG | |
| N2-B3 | GACTTGATCTTTGAAATTTGGATCT | |
| N2-FIP | TTCCGAAGAACGCTGAAGCGGAACTGATTACAAACATTGGCC | |
| N2-BIP | CGCATTGGCATGGAAGTCACAATTTGATGGCACCTGTGTA | |
| N2-LF | GGGGGCAAATTGTGCAATTTG | |
| N2-LB | CTTCGGGAACGTGGTTGACC | |
| N2-QFIP | /5IABkFQ/TTCCGAAGAACGCTGAAGCGGAACTGATTACAAACATTGGCC | |
| N2-FD22 | TCCGCTTCAGCGTTCTTCGGAA/36-FAM/ | |
Modifications as noted in Integrated DNA Technologies’ nomenclature.
BIP: Backward inner primer; E: SARS-CoV-2 E gene; FIP: Forward inner primer; IAV: Influenza A virus primer; IBV: Influenza B virus primer; N: SARS-CoV-2 N gene; QFIP: Quencher-modified forward inner primer.