Table 2.

Screening of PHA-producing archaeal strains using phenotypic and genotypic methods.

Sample type	Name of isolates	Morphology	Colony staining method		CODEHOP PCR		ARDRA
			SBB	NR	PhaE	PhaC	Profiles
Water and sediments (2014)	CEJ1-14	Cocci	-	-	-	-	I
	CEJ2-14	Cocci	-	-	-	-	II
	CEJ3-14	Cocci	+	+	+	+	III
	CEJ4-14	Cocci	-	-	-	-	I
	CEJ5-14	Cocci	+	+	+	+	III
	CEJ6-14	Cocci	+	+	+	+	III
	CEJ7-14	Cocci	+	+	+	+	III
	CEJ8-14	Cocci	+	+	+	+	III
	CEJ9-14	Cocci	+	+	+	+	III
	CEJ10-14	Cocci	+	+	+	+	III
	CEJ11-14	Cocci	+	+	+	+	III
	CEJ17-14	Pleomorph	-	-	-	-	I
	CEJ18-14	Pleomorph	-	-	-	-	I
	CEJ21-14	Cocci	+	+	+	+	III
	CEJ24-14	Cocci	+	+	+	+	III
	CEJ25-14	Cocci	+	+	+	+	III
	CEJ26-14	Cocci	-	-	-	-	I
	CEJ28-14	Cocci	+	+	+	+	III
	CEJ29-14	Short rod	-	-	-	-	I
	CEJ32-14	Cocci	-	-	-	-	II
Water (2014)	CEJ33-14	Cocci	-	-	-	-	I
	CEJ34-14	Cocci	+	+	+	+	I
Water and sediments (2010)	CEJ35-10	Cocci	-	-	-	-	IV
	CEJ36-10	Cocci	-	-	-	-	IV
	CEJ37-10	Cocci	-	-	-	-	IV
	CEJ38-10	Cocci	-	-	-	-	IV
	CEJ41-10	Cocci	-	-	-	-	VI
	CEJ42-10	Cocci	-	-	-	-	VI
	CEJ43-10	Cocci	-	-	-	-	IV
	CEJ45-10	Cocci	-	-	-	-	IV
	CEJ46-10	Cocci	-	-	-	-	IV
	CEJ47-10	Cocci	-	-	-	-	I
	CEJ48-10	Cocci	+	+	+	+	V

Designation of isolates by letters indicating first the origin of strains from Chott El Jerid (CEJ) followed by the number of the isolates, then a number indicating the year of sampling. The ARDRA pattern is indicated by Roman numeral. +, detectable; -, not detectable; SBB: Sudan Black B; NR: Nile Red.