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. 2021 Jan 21;11:542950. doi: 10.3389/fphys.2020.542950

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Copyright © 2021 Haskins, Bhuvanendran, Anselmi, Gams, Kanholm, Kocher, LoTempio, Krohmaly, Sohai, Stearrett, Bonner, Tuchman, Morizono, Jaiswal and Caldovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-immunoprecipitation of NAGS, CPSI, and OTC from the mouse liver. Anti-NAGS (IP NAGS lane), anti-CPSI (IP CPSI lane), and anti-OTC (IP OTC lane) antibodies were used for immunoprecipitation. Non-specific IgG (Neg) was used as a negative control. The precipitated proteins were probed with antibodies against NAGS (A) and CPSI (B). Either 0.5 ng of recombinant NAGS-M and NAGS-C (panel A) or 5 μg (panel B) of liver proteins (Liver lane) were used as positive controls. Between 0.5 and 1 μg of immunoprecipitated proteins were resolved in IP NAGS, IP CPSI, and IP OTC lanes. Graphs on the right represent signal intensities of the bands representing co-immunoprecipitated proteins (colored lines) and their negative controls (gray).