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. Author manuscript; available in PMC: 2021 Feb 4.
Published in final edited form as: Exp Gerontol. 2019 Dec 5;130:110805. doi: 10.1016/j.exger.2019.110805

Figure 4.

Figure 4.

Inhibition of AhR prevents kynurenine-induced senescence in BMSCs. A. beta-galactosidase assay in BMSCs treated with indicated doses of KYN for 24h in the presence or absence of 2μg/ml of AhR inhibitor, CH223191. Data are expressed as β-gal-positive staining area percentage and are representative of at least 3 independent experiments with three replicates for each group. * - p< 0.05 vs. 0μM KYN + vehicle, # - p< 0.05 vs. 100μM KYN + vehicle. B. Expression of p21 in BMSCs treated with indicated doses of KYN 48 hr. with or without 10μM of AhR inhibitor, 3’4’-DMF (DMF). Data are expressed as p21/β-actin ratio adjusted to vehicle-0μM KYN. * - p< 0.05 vs. 0μM KYN, # - p< 0.05 vs. KYN + vehicle. C. Histone 3-methyl-9K nuclear staining (green) in BMSCs treated with indicated doses of KYN for 24h with or without 10μM of 3’4’DMF. Nuclei were stained with DAPI (blue). Images are representative of 3 independent experiments with three replicates for each group.