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. 2020 Jun 8;19(9):3708–3715. doi: 10.1021/acs.jproteome.0c00245

Figure 3.

Figure 3

C. elegans viability and hydrogen peroxide uptake. (A) Percent viability of C. elegans using 200 mM hydrogen peroxide in the presence of 0, 0.1, 0.5, and 1% AZ. Negative control is C. elegans in the presence of 50% methanol, and positive control is C. elegans in M9 buffer. Error bars are calculated across technical triplicates (left). Fluorescence integrated densities (FID) for hydrogen peroxide uptake in the presence of 0–1% AZ (right). (B) Representative C. elegans fluorescence images in response to intercellular hydrogen peroxide: no hydrogen peroxide control (left), 200 mM hydrogen peroxide (middle), and 200 mM hydrogen peroxide with 1% azone (right). (C) Percent viability of C. elegans using 200 mM hydrogen peroxide in the presence of 0, 0.1, 0.5, and 1% OA. Negative control is C. elegans in the presence of 50% methanol, and positive control is C. elegans with no hydrogen peroxide. Error bars are calculated across technical triplicates (left). FID for hydrogen peroxide uptake in the presence of 0–1% OA (right).