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. 2020 Jun 8;19(9):3708–3715. doi: 10.1021/acs.jproteome.0c00245

Figure 5.

Figure 5

In vitro and IV-FPOP using two CPEs. (A) Intact MS analysis of ubiquitin. The peak area for the +16 FPOP modification peak for each 2CPE condition tested is shown. (B) Percent viability of C. elegans using 200 mM hydrogen peroxide in the presence of AZ, OA, and 2CPEs. Negative control C. elegans are in the presence of 50% methanol, and positive control were not exposed to hydrogen peroxide. Error bars are calculated across technical triplicates. (C) FID for hydrogen peroxide uptake in the presence of AZ, OA, and 2CPEs. (D) Oxidatively modified proteins using 200 mM hydrogen peroxide in the presence and absence of 0.5% 2CPEs.