Figure 2: Representative bacterial conjugation results and a schematic of the colistin Tn-seq experiment.

(A) Representative kanamycin selection plate. The plate is divided into five equal sections. Blue dots represent colony counting for estimation of A. baumannii transposon insertion mutants. At least three separate plates were counted to calculate the final estimation. (B) Identification of fitness factors at subinhibitory colistin concentrations. The pooled transposon library was grown to logarithmic growth phase either in the absence (control) or in the presence (experimental) of colistin. Once the cultures reached appropriate optical density, the cells were pelleted and gDNA was extracted from each sample. Each condition was tested in duplicate for a total of four samples. Please click here to view a larger version of this figure.