Skip to main content
. Author manuscript; available in PMC: 2021 Feb 4.
Published in final edited form as: J Vis Exp. 2020 Jul 7;(161):10.3791/61612. doi: 10.3791/61612

Figure 4: Representative quality control (QC) results and map of the plasmid carrying the transposition genes.

Figure 4:

(A) QC trace for a DNA library build. There is a peak at ~ 350 base pairs, indicating successful library build. If some larger DNA was detected in the QC results, the samples can be cleaned up further to remove large DNA fragments. (B) Plasmid pJNW684 consists of a Himar1 mariner transposon (green) with a kanamycin resistance cassette (purple) for mutant selection, a gene encoding the hyperactive mariner Himar1 C9 transposase (red) under control of an A. baumannii 17978 specific promoter (blue), an ampicillin resistance gene (bla, orange) and a RP4/oriT/oriR6K-conditional origin of replication (yellow). Please click here to view a larger version of this figure.