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. 2021 Feb 4;16(2):e0246531. doi: 10.1371/journal.pone.0246531

Fig 11. Identification of critical cysteine residue in HIF-1alpha regulation by PDI.

Fig 11

HIF-1alpha C90S, HIF-1alpha C210S, or HIF-1alpha C219S/3×FLAG-pcDNA4 vector, or PDI WT, or PDI C53, 397S / pcDNA 3.1 (+) vector was transfected into Hep3B cells, and immunoblotting was performed with the anti-FLAG, -PDI, or-beta-actin antibody. The HIF-1alpha protein levels of mock cells under normoxic conditions were set to 1.0. Values are expressed as the mean ± S.D. (error bars) of three different plates. ctrl, control.  * p<0.05; ** p<0.01, significantly different from mock cells.