Table 1. Primers used to clone PDI C53, 397S, HIF-1alpha WT, HIF-1alpha K719T, Ero-1 WT, Ero-1 (24–469 aa), Ero-1 C104, 131S, Ero-1 C394, 397S, HIF-1alpha (1–245 aa), HIF-1alpha (245–826 aa), HIF-1alpha C90S (1–245 aa), HIF-1alpha C210S (1–245 aa), and HIF-1alpha C219S (1–245 aa).
| Factor | Primer no. | Sequence | GenBank |
|---|---|---|---|
| accession number | |||
| PDI | 1 | ATTGGATCCGACATGCTGCGCCGCGCTCTGCTGTGCC | NM_000918 |
| C53, 397S | 2 | AGCCTTGCAGTGGCCACTCCAAGGGGCATAGAA | |
| 3 | CACTGCAAGGCTCTGGCCCCT | ||
| 4 | AATGAATTCGTATTACAGTTCATCTTTCACAGCTTTC | ||
| 5 | CAACTGTTTGCAGTGACCACTCCATGGGGCATAGAA | ||
| 6 | CACTGCAAACAGTTGGCTCCC | ||
| HIF-1alpha | 7 | TTGATATCATGGAGGGCGCCGGCGGCGC | NM_001530 |
| WT | 8 | ATAATTTAGCGGCCGCTCAGTTAACTTG | |
| HIF-1alpha | 9 | TTCTCTGAGCATTCTGCAAA | NM_001530 |
| K719T | 10 | AGAATGCTCAGAGAACGCGAAAAATGGAACA | |
| Ero-1 WT | 11 | AAGGATCCATGGGCCGCGGCTGGGGATT | NM_014584 |
| 12 | TTTCTCGAGTTAATGAATATTCTGTAACA | ||
| Ero-1 | 13 | ACTTCATCAGATTGAGATGGTTTGACAGCAC | NM_014584 |
| C104, 131S | 14 | TCAATCTGATGAAGTTCCTG | |
| 15 | CGTTCAGCTTGTTCAGATTCTTCAATGAGAT | ||
| 16 | TGAACAAGCTGAACGACTTG | ||
| Ero-1 | 17 | CAGACGACATTTAAAAGAACCAACACAATCCAT | NM_014584 |
| C394, 397S | 18 | AAATGTCGTCTGTGGGGAAAG | |
| 19 | TTTCCCCACAGACGAGATTTAAAAGAACCAA | ||
| 20 | CTGTGGGGAAAGCTTCAGACT | ||
| Ero-1 | 21 | AAGGATCCGAGGAGCAGCCCCCGGAGAC | NM_014584 |
| (24–469 aa) | 22 | TTAGTCGACTTAATGAATATTCTGTAACA | |
| HIF-1alpha | 23 | AAGGATCCATGGAGGGCGCCGGCGGCGCGAAC | NM_001530 |
| (1–245 aa) | 24 | TGAATTCTTATCGACTGAGGAA | |
| 25 | ATGGCCATGGAGGCCATGGAGGGCGCCGGCGGCGCG | ||
| 26 | AAGGATCCATGGAGGGCGCCGGCGGCGCGAAC | ||
| 27 | TTGGTACCTCATCGACTGAGGAAAGT | ||
| HIF-1alpha | 28 | ATGGCCATGGAGGCCCGACACAGCCTGGAT | NM_001530 |
| (245–826 aa) | 29 | TTAGCGGCCGCTCAGTTAACTTGATCCAAAG | |
| HIF-1alpha C90S(1–245 aa) | 30 | GGCTTTCAAATAAAAGCTATTCATCTGTGCTTT | NM_001530 |
| 31 | TATTTGAAAGCCTTGGATGGT | ||
| HIF-1alpha C210S(1–245 aa) | 32 | TGGTTTCTTATACCCACTCTGAGGTTGGTTACT | NM_001530 |
| 33 | TATAAGAAACCACCTATGACC | ||
| HIF-1alpha C219S(1–245 aa) | 34 | ACAAATCAGCACCAAGCTGGTCATAGGTGGTTT | NM_001530 |
| 35 | GTGCTGATTTGTGAACCCATT |
Restriction sites were indicated as underlined, start codon (ATG) and stop codons (TTA or TCA) were indicated as bold characters, and mutant codons were indicated as shadow and bold characters.