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Stress-induced microspore embryogenesis in B. napus. Micrographs of toluidine blue-stained sections of (A) isolated vacuolated microspore, before stress (B) proembryo of stress-treated microspore culture, (C) globular embryo and (D) cotyledonary embryo. (E) Petry dish containing microspore-derived embryos and (F) higher magnification of cotyledonary embryos. Ex: exine; N: nucleus; V: vacuole. Scale bars: (A–C): 10 �m; (D): 500 �m; (E): 10 mm; and (F): 1 mm.
