Fig. 3.

MCA activity in microspore embryogenesis. Effect of pH (A), EDTA (B) and protease inhibitors at 10 �M (C) on VRPRase activity of protein extracts from microspore-derived embryos and recombinant mcII-Pa. The data represent mean � SEM. Different letters indicate significant differences within protein extracts or recombinant mcII-Pa series (Tukey’s t-test at P < 0.05). Quantification of cell death in control and Z-VRPR-FMK-treated microspore cultures, at two stages: stress-treated microspore culture (D) and 10-day culture (E). Different letters indicate significant differences (chi-square test at P < 0.05).