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. 2020 Oct 15;61(12):2097–2110. doi: 10.1093/pcp/pcaa128

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� The Author(s) 2020. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 6 — Electron microscopy analysis of autophagosomes and ATG8 localization in microspores. (A) Cytoplasmic area of vacuolated microspore, before stress. (B) Cytoplasmic area of stress-treated microspore treated with E-64d to preserve autophagic bodies in the vacuoles (arrows). (C) Autophagosome in stress-treated microspore. (D and E) Immunogold labeling of ATG8 (arrows) is restricted to autophagosomes in stress-treated microspores. V, vacuole; M, mitochondria; S, starch granule. Scale bars: (A, B) 1 �m; (C–E) 0.2 �m.