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. 2021 Feb 4;16(2):e0246630. doi: 10.1371/journal.pone.0246630

Fig 7. Requirement of PAK2 for IL-31-induced neurite outgrowth and STAT3 phosphorylation.

Fig 7

Neuro2A cells were transfected with siRNA for PAK2 or control siRNA. PAK2 expression was determined by immunoblotting (A). Representative images of Neuro2A cells after transfection with siRNA for PAK2 or control siRNA. Neurites were defined as a process with lengths equivalent to one diameter of a cell body (B). The percentage of neurite-bearing cells was calculated from the total number of counted cells. Scale bar: 20 μm. Phosphorylated STAT3 at Tyr705 was analyzed by immunoblotting (C). Neuro2A cells were treated with rIL-31 or anti-IL-31Rα antibody in the absence or presence of siRNA for PAK2, and the cell lysates were examined by immunoblotting with indicated antibodies. The representative images are shown (upper), and the quantitative data of the ratios of phosphorylated STAT3 versus STAT3 are shown (lower). Data are pooled from three independent experiments and shown as mean and SD. **p < 0.01 as compared with control; ##p < 0.01 as compared with rIL-31-treated group; $ $p < 0.01 as compared with anti-IL-31Rα antibody-treated group (one-way ANOVA/Tukey-Kramer post-hoc comparisons).