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. 2020 Nov 27;9:e61591. doi: 10.7554/eLife.61591

Figure 3. Dopamine neuron stimulation between reaches does not affect skilled reaching performance.

(A) Average number of trials per session divided by the baseline number of trials for ‘between reach’ stimulation. Gray lines represent individual rats. Linear mixed model: effect of laser: t(79) = 1.13, p=0.26; interaction between laser and session: t(584) = −0.64, p=0.52. (B) Average first attempt success rate divided by baseline success rate for ‘between reach’ stimulation. Linear mixed model: effect of laser: t(133) = −0.29, p=0.78; interaction between laser and session: t(584) = −0.94, p=0.35. (C) Moving average of success rate within individual sessions in the last retraining session, first 5 ‘laser on’ sessions, and first 5 ‘occlusion’ sessions. ‘R10’, ‘L1’, ‘O1’, etc. indicate the 10th retraining session, first ‘laser on’ session, first ‘occlusion’ session, etc. ‘During reach’ data from Figure 2 are shown for comparison. Unlike panel B, these are not normalized to retraining sessions because they are within-session moving averages. Black bars represent trials with a statistically significant difference between groups (Wilcoxon rank sum text, p<0.01). Data for individual rats are shown in Figure 3—figure supplement 1. Figure 3—figure supplement 2 shows additional performance measures for ‘between reach’ ChR2 activation (‘any reach’ success rates, number of attempts per trial, and breakdown of reach outcomes across sessions). Shaded colored areas in C and error bars in A-B represent s.e.m.

Figure 3—source data 1. A .mat file containing number of trials (num_trials) and first reach success rate (firstReachSuccess) for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
Data is average per session for each rat. The field ‘experimentInfo’ provides information on groups.
Figure 3—source data 2. A .mat file containing first reach success rate averages across a moving block of 10 trials for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
The field ‘exptInfo’ provides information on groups.
Figure 3—source data 3. Statistics.
A .xlsx file containing the statistical output of Wilcoxon ranksum tests for comparisons between ChR2 During and ChR2 Between group averages in Figure 3C.

Figure 3.

Figure 3—figure supplement 1. Individual rat data for moving average of success rate across trials within individual sessions (‘ChR2 Between’).

Figure 3—figure supplement 1.

Each colored line represents the same rat across panels. ‘R10’, ‘L1’, ‘O1’, etc. indicate the 10th retraining session, first ‘laser on’ session, first ‘occlusion’ session, etc.
Figure 3—figure supplement 1—source data 1. A .mat file containing first reach success rate averages across a moving block of 10 trials for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
The field ‘exptInfo’ provides information on groups.
Figure 3—figure supplement 2. Additional task performance measures for ‘between reach’ ChR2 stimulation.

Figure 3—figure supplement 2.

(A) ‘Any reach’ success rate. Linear mixed model: effect of laser: t(144) = −0.31, p=0.76; interaction between laser and session: t(585) = −0.55, p=0.58. (B) Number of reach attempts per trial. Linear mixed model: effect of laser: t(67) = 0.46, p=0.64; interaction between laser and session: t(585) = 0.29, p=0.77. (C) Breakdown of trial outcomes by session (see Materials and methods, Number of trials and success rate for definitions of the outcomes). There was a significant increase in ‘no pellet’ (linear mixed model: effect of laser: t(607) = 2.85, p=4.45×10−3; interaction between laser and session: t(612) = −2.85, p=4.59×10−3) and ‘laser error’ (linear mixed model: effect of laser: t(389) = −0.94, p=0.35; interaction between laser and sessions: t(612) = 2.15, p=0.03) trials during ‘laser on’ sessions. All other outcomes were consistent across sessions. * indicates p<0.05 and ** indicates p<0.01 in the linear mixed model in panel C. Purple box in Panel E highlights ‘Laser On’ sessions.
Figure 3—figure supplement 2—source data 1. A .mat file containing any reach success rate (anyReachSuccess) and number of reach attempts (mean_num_reaches) for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
Data is average per session for each rat. The field ‘experimentInfo’ provides information on groups.
Figure 3—figure supplement 2—source data 2. A .mat file containing percentage of trials that were each outcome type for each session for each rat (fullOutcomePercent).
The field ‘fullOutcomeNames’ lists the names of each outcome type (see Materials and methods for descriptions). The field ‘experimentInfo’ provides information on groups.
Figure 3—figure supplement 2—source data 3. Statistics.
A .xlsx file containing the statistical output of linear mixed-effects models run for each outcome type (Figure 3—figure supplement 2C). Statistics are reported for effects of laser and interactions between laser and session number.