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. 2020 Nov 27;9:e61591. doi: 10.7554/eLife.61591

Figure 4. Dopamine neuron inhibition during reaches decreases the number of reaches performed but does not impair reach accuracy.

(A) Average number of trials per session divided by the baseline number of trials for ‘during reach’ inhibition. Gray lines represent individual rats. Linear mixed model: effect of laser: t(80) = −0.21, p=0.84; interaction between laser and session: t(584) = −2.64, p=8.47×10-3. (B) Same as (A) for ‘between reach’ inhibition. Linear mixed model: effect of laser: t(80) = 0.93, p=0.36; interaction between laser and session: t(584) = −1.52, p=0.13. (C) Average first attempt success rate divided by baseline success rate for ‘during reach’ inhibition. Linear mixed model: effect of laser: t(133) = 0.59, p=0.56; interaction between laser and session: t(584) = −0.40, p=0.69. (D) Same as (C) for ‘between reach’ inhibition. Linear mixed model: effect of laser: t(133) = −0.64, p=0.52; interaction between laser and session: t(584) = 0.10, p=0.92. (E) Moving average of success rate across trials within individual sessions in the last retraining session, first 5 ‘laser on’ sessions, and first 5 ‘occlusion’ sessions. Unlike panels C and D, these are not normalized to retraining sessions because they are within-session moving averages. Black bars represent statistically significant differences between groups (Wilcoxon rank sum test, p<0.01). ‘R10’, ‘L1’, ‘O1’, etc. indicate the 10th retraining session, first ‘laser on’ session, first ‘occlusion’ session, etc. Figure 4—figure supplement 3 shows additional performance measures for ‘during reach’ and ‘between reach’ Arch activation (‘any reach’ success rates, number of attempts per trial, and breakdown of reach outcomes across sessions). Shaded colored areas in (E) and error bars in A-D represent s.e.m. Moving average of success rate within sessions for Arch Between rats is shown in Figure 4—figure supplement 1. Data for individual Arch During and Arch Between rats are shown in Figure 4—figure supplement 2. ** indicates p<0.01 for the laser-session interaction term in panel A.

Figure 4—source data 1. A .mat file containing number of trials (num_trials) and first reach success rate (firstReachSuccess) for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
Data is average per session for each rat. The field ‘experimentInfo’ provides information on groups.
Figure 4—source data 2. A .mat file containing first reach success rate averages across a moving block of 10 trials for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
The field ‘exptInfo’ provides information on groups.
Figure 4—source data 3. Statistics.
A .xlsx file containing the statistical output of Wilcoxon ranksum tests for comparisons between Arch During and EYFP group averages in Figure 4E.

Figure 4.

Figure 4—figure supplement 1. Moving average of success rate across trials within individual sessions in Arch-injected rats receiving dopamine neuron inhibition between reaches.

Figure 4—figure supplement 1.

‘During reach’ data from Figure 4 are shown for comparison. ‘R10’, ‘L1’, ‘O1’, etc. indicate the 10th retraining session, first ‘laser on’ session, first ‘occlusion’ session, etc.
Figure 4—figure supplement 1—source data 1. A .mat file containing first reach success rate averages across a moving block of 10 trials for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
The field ‘exptInfo’ provides information on groups.
Figure 4—figure supplement 1—source data 2. Statistics.
A .xlsx file containing the statistical output of Wilcoxon ranksum tests for comparisons between Arch During and Arch Between group averages in Figure 4—figure supplement 1.
Figure 4—figure supplement 2. Individual rat data for moving average of success rate across trials within individual sessions (‘Arch During’ and ‘Arch Between’).

Figure 4—figure supplement 2.

Each colored line represents the same rat across panels. ‘R10’, ‘L1’, ‘O1’, etc. indicate the 10th retraining session, first ‘laser on’ session, first ‘occlusion’ session, etc.
Figure 4—figure supplement 2—source data 1. A .mat file containing first reach success rate averages across a moving block of 10 trials for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
The field ‘exptInfo’ provides information on groups.
Figure 4—figure supplement 3. Additional task performance measures for ‘during reach’ and ‘between reach’ Arch activation.

Figure 4—figure supplement 3.

(A) ‘Any reach’ success rate for ‘during reach’ Arch activation. Linear mixed model: effect of laser: t(144) = 0.32, p=0.75; interaction between laser and session: t(585) = −0.13, p=0.89. (B) Same as (A) for ‘between reach’ Arch activation. Linear mixed model: effect of laser: t(144) = −0.15, p=0.88; interaction between laser and session: t(585) = −0.41, p=0.69. (C) Number of reach attempts per trial for ‘during reach’ Arch activation. Linear mixed model: effect of laser: t(67) = −0.73, p=0.47; interaction between laser and session: t(585) = −0.42, p=0.68. (D) Same as (C) for ‘between reach’ Arch activation. Linear mixed model: effect of laser: t(67) = 0.36, p=0.72; interaction between laser and session: t(585) = −0.46, p=0.65. (E) Breakdown of trial outcomes by session for ‘during reach’ Arch activation (left) and ‘between reach’ Arch activation (right) (see Materials and methods, Number of trials and success rate for definitions of the outcomes). All outcomes remained consistent across sessions for both groups. Green and orange boxes highlight ‘Laser On’ sessions in Panel E.
Figure 4—figure supplement 3—source data 1. A .mat file containing any reach success rate (anyReachSuccess) and number of reach attempts (mean_num_reaches) for 22 testing sessions (‘retraining’, ‘laser on’, and ‘occluded’).
Data is average per session for each rat. The field ‘experimentInfo’ provides information on groups.
Figure 4—figure supplement 3—source data 2. A .mat file containing percentage of trials that were each outcome type for each session for each rat (fullOutcomePercent).
The field ‘fullOutcomeNames’ lists the names of each outcome type (see Materials and methods for descriptions). The field ‘experimentInfo’ provides information on groups.
Figure 4—figure supplement 3—source data 3. Statistics.
A. xlsx file containing the statistical output of linear mixed-effects models run for each outcome type (Figure 4—figure supplement 3E). Statistics are reported for effects of laser and interactions between laser and session number.