Skip to main content
. 2021 Feb;21(1):98–110. doi: 10.17305/bjbms.2020.4639

FIGURE 1.

FIGURE 1

(A) Morphological characteristics and population doubling time of undifferentiated 46C cells. Good quality 46C cells exhibit a high nucleus-to-cytoplasm ratio. The doubling time for 46C cells was 20.0 ± 2.07 h and is representative of randomly chosen passages (n = 12). (B) Immunostaining and flow cytometry analysis of pluripotency-associated protein markers: Oct4, SSEA1, and Nanog. Blue shows nuclear counterstain with DAPI, while green shows expression of Oct4 and SSEA1; red shows expression of Nanog. Flow cytometry analysis of 46C cells for Oct4, SSEA1, and Nanog. The percentage of fluorescent intensity of gated treated cells is shown on the histograms. (C) 46C cell line was able to generate good quality EBs that presented with cavitation process (center), smoothness of the boundary, and acceptable diameter (308.18 ± 28.16 mm; and is representative of randomly chosen passages; n = 12). The scale bars represent 100 mm (A) and (C); and 200 mm (B) for micrographs. Oct4: Octamer-binding transcription factor 4; SSEA1: Stage-specific embryonic antigen 1.