FIGURE 3.

Immunocytochemical analysis was performed to evaluate the expression of neural and glial cell proteins after neural differentiation of 46C cells on day 5 neuron post-plating on PDL/laminin-coated plate. The expression of (A) post-mitotic neurons (class III β-tubulin); (B) mature neurons (MAP2); (C) neurofilament; and (D) astrocytes (GFAP) was observed in the neuron cultures. Green indicates the expression of protein markers; meanwhile, nuclei were counterstained with PI (red) or DAPI (blue). Scale bar is 100 mm. PDL: Poly-D-Lysine; MAP2: Microtubule-associated protein 2; GFAP: Glial fibrillary acidic protein; PI: Propidium iodide.