Changes in HDL apolipoprotein levels after surgery. HDL fractions were isolated from patient plasma (n = 13) before (PRE) and just after (POST) orthopedic surgery and on postoperative days (POD) 1, 3, and 6 or 7 by ultracentrifugation. Fractions were resolved by SDS-PAGE (4 μg/lane) followed by CBB staining. A representative profile is shown (a). Bands at 28, 12, and 8 kDa represent apoA-I, SAA, and apoA-II, respectively. Band intensities were semiquantified by densitometry. The intensities for apoA-I (b), SAA (c), and apoA-II (d) are shown as percentages of the summed intensity for all three bands. Values represent the mean, min, and max. ∗p < 0.05, ∗∗p < 0.005, and ∗∗∗p < 0.001 vs. POST by paired t-test.