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. 2019 Nov 20;9(22):e3434. doi: 10.21769/BioProtoc.3434

Figure 2. Inoculation with C. higginsianum.

Figure 2.

A. Setup to separate mycelia from conidia. B. Scraping the fungal culture using a painting brush to obtain conidia suspension. C. Filtering the conidia suspension through nylon mesh. D. Conidial pellet after centrifugation. E. Conidia on a hemacytometer (Black arrows). Bar = 100 μm. F. Inoculation of conidia on the leaf surface using a pipette.