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. 2019 Nov 20;9(22):e3434. doi: 10.21769/BioProtoc.3434

Figure 3. Data analysis using color thresholds in ImageJ.

Figure 3.

. A. Setting regions of interest (ROIs) on a photo. Yellow rectangles indicate ROIs that will be measured with color thresholds. Arrow heads indicate the rectangle tool and the straight tool. B. The Threshold Color menu of ImageJ. Red-colored parts in ROIs will be detected as lesions. C. Lesion.ijm opened in a text editor indicating the lines specifying the minimum and maximum thresholds of hue, saturation, and brightness, respectively. D. An expected result using the sample files provided in this article.