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. 2020 Dec 11;40(5):980–996. doi: 10.1038/s41388-020-01576-6

Fig. 5. miR-375 antagomirs in MCC cells diminish coculture induced fibroblast polarization.

Fig. 5

MRC-5 cells (a, b, e, g, i) or Fibro 1.12 primary skin fibroblasts (c, d, f, h, j) were directly cocultured with WaGa or PeTa cells transfected with miR-375 antagomirs for 72 h. a, c The relative miR-375 expression level in fibroblasts under the indicated conditions was determined by RT-qPCR. b, d α-SMA protein expression in fibroblasts under the indicated conditions was determined by immunoblotting; β-tubulin served as loading control. e, f The relative ACTA2, CXCL2 and IL1B expression level in fibroblasts under the indicated conditions was determined by RT-qPCR. g, h Relative expression of TP53 mRNA (left) and protein (right) level in fibroblasts under the indicated conditions was determined by RT-qPCR and immunoblotting. i, j Relative expression of RBPJ mRNA (left) and protein (right) level in fibroblasts under the indicated conditions was determined by RT-qPCR and immunoblotting. For all RT-qPCR experiments, Cq values were normalized to HPRT mRNA expression and compared to the ΔCq of the corresponding untreated MRC-5 or Fibro.1.12 cells transfected. Experiments were biologically replicated trice and were performed in triplicates. The error bars indicate the SDs; * indicates p < 0.05, ** indicates p < 0.01.