Fig. 4. Monocyte depletion ameliorates BM fibrosis and anemia in mice transplanted with Jak2V617F BMCs.

a Representative images of HE, reticulin silver, and Masson trichrome staining of BM sections from recipient mice transplanted with Jak2V617F/CD11b-DTR BMCs and those transplanted with CD11b-DTR BMCs (n = 3 in each group). Bars: (HE) 50 μm; (reticulin) 50 μm; (trichrome) 50 μm. b The total numbers of BM nucleated cells in one femur and one tibia. c The number of megakaryocytes is presented as the average of 10 random HPFs from three mice of each type. d The proportion of LSKs (Lin⁻Sca-1⁺ c-Kit⁺), long-term HSCs (CD150⁺48⁻Lin⁻Sca-1⁺c-Kit⁺), short-term HSCs (CD150⁻48⁻Lin⁻Sca-1⁺c-Kit⁺), MPPs (CD150⁻48⁺Lin⁻Sca-1⁺c-Kit⁺), CMPs (IL-7Rα⁻Lin⁻c-Kit⁺Sca-1⁻FcγRloCD34⁺), GMPs (IL-7Rα⁻Lin⁻c-Kit⁺Sca-1⁻FcγR⁺CD34⁺), MEPs (IL-7Rα⁻Lin⁻c-Kit⁺Sca-1⁻FcγRloCD34⁻), and MKPs (CD9⁺CD41⁺FcγRloc-kit⁺Lin⁻) in BMCs was analyzed by flow cytometry. e FACS analysis of Mac1⁺Gr1⁺ granulocytes, Mac1⁺Gr1⁻ monocytes, CD3⁺ T cells, B220⁺ B cells, CD71⁺Ter119⁺ early erythroblasts, CD71⁻Ter119⁺ late erythroblasts, and CD41⁺ megakaryocytes in BM. f The absolute number of CD71⁺Ter119⁺ early erythroblasts and, CD71⁻Ter119⁺ late erythroblasts per mouse. Six mice were analyzed in group A and B, and eight mice were analyzed in group C and D (b, d−f). Data are expressed as means ± SEM. One-way ANOVA followed by the Tukey–Kramer test was used (b–f). ***P < 0.001, **P < 0.01, *P < 0.05, ^☨☨P < 0.01. n.s. not significant. g Peripheral blood counts in each group (n = 12 in group A and C, n = 14 in group B and D) during 8-week treatment with PBS or DT. Data are expressed as means ± SEM. ANOVA with repeated measures was used. **P < 0.01, *P < 0.05, ^☨P < 0.05. n.s. not significant.