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The five flavonoids were able to suppress HSC activation. HSC-T6 cells were stimulated with TGF-β1 (10 ng/ml) for 24 h and prepared for further experiments. (A) Alpha-SMA protein expression in TGF-β1-induced HSC-T6 cells (n = 3). (B) Confocal scanning images and quantified data of α-SMA in HSC-T6 cells stimulated with TGF-β1 (10 ng/ml) for 24 h (n = 3), scale bar = 50 μm for 400×. Data are expressed as the mean ± SD. *p < 0.05 vs. TGF-β1 treatment.
