Figure 6.

Detection of N protein in Fostera PRRSV–infected IPKM. IPKM were treated with or without 80 ng/ml DEX for 1 d in the presence or absence of 10 μM SB203580. DMSO (0.1%) was used as a vehicle control because SB203580 was dissolved in DMSO. Then, the cells were exposed to the Fostera PRRSV for 2 h, cultured for a further day, and lysed with extraction buffer. The N protein was detected in the Fostera PRRSV-exposed IPKM, even in the presence of DMSO. DEX treatment had no effect on the expression of the N protein, while SB203580 clearly reduced it. The experiments were performed in triplicate and produced similar results (upper three panels). An anti-actin antibody was used as a loading control for the immunoblotting (the lowest panel).