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. 2020 Sep 8;28(2):671–684. doi: 10.1038/s41418-020-00616-8

Fig. 1. UBE2O interacts with Mxi1 in vitro and in vivo.

Fig. 1

a HEK293T cells transfected with indicated plasmids for 24 h were treated with MG132 (10 μM) for 4 h. Cells were lysed with NETN buffer and then analyzed by co-IP with S beads or Myc agarose followed by western blotting (n = 3). b HeLa cells were transfected with SFB-Mxi1 or SFB-UBE2O for 24 h and treated with MG132 (10 μM) for 4 h. Cells were collected for co-IP with S beads followed by western blotting (n = 3). c HeLa, H1299, and A549 cell were treated with MG132 (10 μM) for 4 h and then harvested. Cell lysates were analyzed by co-IP with anti-IgG, Mxi1, or UBE2O antibodies followed by western blotting (n = 3). d Recombinant GST-only and GST-Mxi1 protein were purified from BL21 bacteria. Cell lysates from HEK293T cells transfected with SFB-UBE2O were incubated with GST or GST-Mxi1 proteins coupled to GST beads overnight and then detected by immunoblotting (n = 3).