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. 2021 Feb 4;11:3063. doi: 10.1038/s41598-021-82341-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of human AFSCs: (a) cells showing adherent growth, exhibiting fibroblast-like aspect (400 ×); (b) differentiation potential in mesenchymal lineage after culture in specific medium (400 ×): adipogenic, oil Red O staining indicating lipidic vesicles; osteogenic, alizarin red staining indicating calcium matrix formation and chondrogenic, Alcian blue staining evidencing glycosaminoglycan presence; (c) immunophenotype analysis of AFSCs by flow cytometry; (d) percentage of cells showing specific markers of MSC, hematopoietic, multipotency, and chondrogenic potential in different samples.