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. 2021 Feb 4;11:3063. doi: 10.1038/s41598-021-82341-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chondrogenic differentiation of CD117⁺ AFSCs seeded into CX scaffold for 21 days with and without IGF-1 stimulation. In the group stimulated by IGF-1 (b,d,f,h,j,l) more compact agglomeration of the cells is observed,with a higher amount of extracellular material compared to that noticed in the non-stimulated control group (a,c,e,g,i,k). H&E staining (a,b); collagen (indicated with *) stained in blue by Masson´s trichrome (MT) (c,d); collagen fibers shown in red by Picrosirius red (PR) (e,f); glycosaminoglycans (‡) stained light blue using Alcian blue (AB) (g,h). Labeling with anti-collagen type II antibody (COL II) (i,j) and anti-aggrecan antibody (ACAN) (k,l) in the stimulated group compared to the control group.