Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 4;11:110. doi: 10.1038/s41398-021-01212-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a GFP-BMAL1 and Flag-DISC1 were co-transfected into HEK293 cells. Empty Flag vector was used as a control. b Quantification of GFP-BMAL1 levels from (a) relative to tubulin control (n = 5, biological replicates). c Endogenous BMAL1 protein expression was decreased in Disc1 knockout (Disc1-LI) MEFs. d Quantification of BMAL1 protein levels from (c) with tubulin control (n = 5, biological replicates). e Endogenous BMAL1 protein levels were measured in MEFs following treatment with 1 μM of dexamethasone to synchronize the circadian cycle. f Quantification of BMAL1 protein levels from (e) relative to tubulin control (n = 3 for each time point, biological replicates). The ratios of the relative band intensity at each time point to the band intensities averaged across all time points were presented. g–j mRNA levels of circadian genes measured by qRT-PCR after dexamethasone synchronization (n = 3 for each time point, biological replicates). The expression of circadian genes was reduced in Disc1 knockout MEFs. The panels show levels of Per1 (g), Per2 (h), Cry1 (i), and Bmal1 (j). Data are means with SEM. *p ≤ 0.05, **p ≤ 0.01, ^#p ≤ 0.0001, unpaired t-test for (b, d); two-way ANOVA and Sidak’s multiple comparisons for (f–i), and (j).