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. 2021 Feb 4;5:4. doi: 10.1038/s41698-021-00144-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–c The joy plot shows the distribution of zygosity of TP53 (a), FLT3 (b), and SRSF2 (c) in T1, T2, and T3, respectively. d The results of Sanger sequencing on single cells (SC) and colonies obtained from PB CD34⁺ cells. On the left are shown the electropherograms representing wild-type, heterozygous, and homozygous SRSF2 gene variants in SC, while on the right there are electropherograms from colonies indicating the heterozygous and homozygous mutational state. In e–f, representative dot plots show genotyping results obtained from SC and colonies. Allele 1 (x) indicates SRSF2 mutated gene and allele 2 (y) refers to SRSF2 wild-type gene. The graph represents the distribution of amplified DNA according to a wild-type (y/y, blue dot), heterozygous (x/y, green dot), and homozygous (x/x, red dot) configuration. In e, the dot plots highlight the presence of wild-type (blue), heterozygous (green), and homozygous (red) single cells. In f, the dot plot shows heterozygous (green) and homozygous (red) colonies.