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. 2021 Feb 4;11:3049. doi: 10.1038/s41598-021-82614-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Efficacy and immune response elicited by BCG/BEAP. (A) The cytokine secretion profile in the supernatant of M.tb antigen stimulated T cells and when activated T cells were co-cultured with M.tb infected macrophages from the respective animals. Dots indicate data points and bars are median with 95% confidence interval. (B) Mycobacterium colony-forming units obtained after M.tb infected macrophages and activated T cells were co-cultured. The data demonstrate a substantial decrease in the mycobacterium CFU in the BEAP and BCG immunization compared to control animals. Dots indicate CFU counts calculated from multiple plates and bars are mean ± SD and analysis was done using two-way ANOVA with Tukey’s correction for multiple comparisons. Graphs were plotted using GraphPad Prism version-7 program (https://www.graphpad.com/).