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. 2021 Feb 1;12(2):138. doi: 10.1038/s41419-021-03413-4

Fig. 5. SNHG1/hnRNPL complex plays a vital role in PCa metastasis via stabilizing CDH1 mRNA.

Fig. 5

A CatRAPID fragment prediction revealed that the sequence of SNHG1 at 400–600 bp had a significant binding possibility with hnRNPL (http://service.tartaglialab.com/page/catra-pid_group). B Schematic diagram of 400–607 bp truncated mutation in SNHG1. C Relative quantitative detection of truncated mutation and wild-type SNHG1 overexpression by qRT-PCR. D HnRNPL RIP confirmed that knockdown SNHG1 reduced the binding ability of hnRNPL to CDH1, and the overexpression of wild-type SNHG1 could restore the binding ability, while the SNHG1-mut with poor binding ability of hnRNPL could not rescue hnRNPL to capture CDH1. E, F Effects of overexpression of wild type SNHG1 or mutant SNHG1 on cell migration by wound healing and migration assays. G, H Knockdown of hnRNPL suppressed the mRNA and protein expression of CDH1(E-cadherin). I HnRNPL knockdown in DU145 cells downregulated CDH1(E-cadherin) mRNA abundance, while ActinomycinD (2.0 μg/ml) were used to inhibit RNA synthesis. All data are shown as the mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-tailed Student’s t test.